Supplementary Figure 5: Peyer’s patches are affected only marginally by the ablation of FRCs.
From: B cell homeostasis and follicle confines are governed by fibroblastic reticular cells
(a) Confocal analysis of Peyer’s patches sections from Ccl19-Cre x Rosa26-EYFP mice stained for GFP, PDPN and RANK-L. Scale bar 150 μm (n=3 mice from two independent experiments). (b,c) Peyer’s patches from Ccl19-Cre x iDTR mice were processed into single cell suspensions and stromal and B cells were enumerated using flow cytometry 72 h after DTxn administration. The figure shows two combined experiments (mean+/-sd, n=3 mice/group per experiment). (d) Confocal analysis of Peyer’s patches from Ccl19-Cre x iDTR animals 72 h following DTxn administration. Scale bars 400 μm (n=3 mice from two independent experiments). NS non significant, *p<0.05
