Supplementary Figure 1: Generation and characterization of Ccl19-Cre × Rosa26-eYFP and Ccl19-Cre × iDTR mice.
From: B cell homeostasis and follicle confines are governed by fibroblastic reticular cells
(a) EYFP expression is achieved in Ccl19-Cre x Rosa26-EYFP mice following Cre-mediated excision of a loxP-flanked transcriptional “stop” sequence. (b) Representative flow cytometric profile of freshly isolated stromal cells from skin draining lymph nodes (n>10 mice from 3 independent experiments). (c) In Ccl19-Cre x iDTR mice, the DTxn receptor gene is expressed following Cre-mediated recombination. (d) Confocal analysis of popliteal lymph nodes from DTR+Cre+ animals 24 h following DTxn administration stained for IgD, RANK-L and MadCAM. Scale bars 100 μm (n=3 mice from 3 independent experiments). (e) Lymph nodes from Ccl19-Cre x iDTR mice were collected, lysed in trizol and analyzed for the presence of Rank-l transcript. Lines indicate mean. Data are normalized to cyclophillin. Each data point represents one lymph node from individual mice (n=4-10 mice/group from 2-3 separate experiments). NS non significant, * p<0.05
