Supplementary Figure 2: Injection of DTxn does not result in systemic toxicity or inflammation.
From: B cell homeostasis and follicle confines are governed by fibroblastic reticular cells
(a) Animal weight was determined following DTxn administration (n=3-4 mice/group per experiment from two independent experiments). (b) Lymph nodes from Ccl19-Cre x iDTR mice were collected and processed into single cell suspensions. Neutrophils (CD45+CD11b+Gr1high), monocytes (CD45+CD11b+Gr1–/low) and dendritic cells (CD45+CD11c+MHCII+) were enumerated using flow cytometric analysis. Data are representative of at least three independent experiments (mean+/-sd, n=3 mice/group per experiment). (c) Lymph nodes from Ccl19-Cre x iDTR mice were collected, lysed in trizol and analyzed for expression of inflammatory mediator genes. Lines indicate mean. Data are normalized to cyclophillin. Each data point represents one lymph node from individual mice (n=4-10 mice/group from 2-3 separate experiments). NS non significant, *p<0.05, **p<0.01, ***p<0.001
