Figure 1: SETX suppresses the expression of genes encoding antiviral molecules, during infection.
From: Senataxin suppresses the antiviral transcriptional response and controls viral biogenesis
(a) Expression of genes encoding known cytokines and ISGs (left margin) in A549 cells left untransfected (No si) or transfected with siSETX, assessed early (4 h) after infection with PR8NS1, presented relative to that in A549 cells transfected with siCtrl. P < 0.01 (analysis of variance (ANOVA) with post-hoc TUKEY HSD test). (b–d) Gene expression (vertical axes) in A549 cells transfected with siRNA as in a, assessed after mock infection (Mock) or infection with PR8NS1 (Inf) (b) or after no treatment (−) or treatment with (+) exogenous IFN-β (c) or TNF (d); results are presented relative to those of infected cells left untransfected (b) or untransfected cells treated with IFN-β1 (c) or TNF (d). (e) Gene expression (vertical axes) in human STAT1-deficient (STAT1-def) and STAT1-sufficient (WT) human fibroblast cells mock infected or infected with PR8ΔNS1 and transfected with siRNA as in a, assessed 4 h after infection; results are presented relative to those of infected wild-type cells left untransfected. *P < 0.05, **P < 0.005 and ***P < 0.0005 (t-test). Data are from three independent experiments (mean and s.d. in b–e).
