Figure 4: TAF4 controls the expression of genes encoding antiviral molecules.
From: Senataxin suppresses the antiviral transcriptional response and controls viral biogenesis
(a) ChIP analysis of RNAPII and SETX the transcriptional start of IFIT1, IFIT2 and GAPDH in uninfected (0 h) or PR8ΔNS1-infected (4 h) A549 cells left untreated (No si) or treated with control siRNA (siCtrl) or TAF4-specific siRNA (siTAF4). (b) Gene expression (vertical axes) in A549 cells treated with siRNA as in a, assessed after mock infection or infection with PR8ΔNS1; results are presented relative those of infected cells without siRNA. (c) Gene expression (vertical axes) in A549 cells treated with siRNA as in a, transfected with empty vector or vector for the overexpression of wild-type TAF4 (TAF4 WT), and mock infected or infected with PR8ΔNS1; results are presented relative to those of infected, siCtrl-treated cells transfected with empty vector. (d) Gene expression (vertical axes) in A549 cells with (+) or without (−) transient expression of SETX or daIRF3 (horizontal axis) and left untransfected (UT) or transfected to overexpress wild-type TAF4 (TAF4 WT) or TAF4 lacking the homology domain required for activator-dependent gene expression (TAF4ΔTAFH) (key); results are presented relative to those of untransfected cells. *P < 0.05, **P < 0.005 and ***P < 0.0005, versus siCtrl-treated cells (a–c) or the corresponding daIRF3-overexpressing cells (d) (t-test). Data are from three independent experiments (mean and s.d.).
