Supplementary Figure 5: Systemic reduction in the T_eff cell population in the presence of STAT5b-CA⁺ T_reg cells.

(a–d) Analysis of Foxp3^Cre-ERT2 (black dots) and Foxp3^Cre-ERT2Rosa26^Stat5bCA (blue dots) mice three months after a single tamoxifen treatment. (a, b) The frequencies of Ki-67⁺ (upper graphs), CD62L^hiCD44^lo (middle; % T naive), and CD62L^loCD44^hi (lower; % T_eff) cells among CD4⁺Foxp3⁻ (a) and CD8⁺Foxp3⁻ (b) cells of the indicated organs. (c) Cytokine production by CD4⁺Foxp3⁻ cells in the spleen and mesenteric LN cells stimulated with anti-CD3/CD28 for 5 hr. (d) Serum Ig levels determined by ELISA. (e) The numbers and sizes of polyps in the distal small intestines of 5-month-old Foxp3^Cre-ERT2Apc^Min/+ and Foxp3^Cre-ERT2Rosa26^Stat5bCAApc^Min/+ mice treated with tamoxifen at 4 wk of age. Cells were analyzed by flow cytometry (a–c). *, P < 0.05; **, P < 0.01; ***, P < 0.001 (two-tailed unpaired Student’s t test). Data are from one experiment representative of three or more independent experiments with similar results with three or more mice per group in each experiment (a–c) or pooled samples with n=10 or more were analyzed (d, e). Each dot represents a single mouse (a–e). mean ± s.e.m. (a–e).