Supplementary Figure 3: Effects of in vivo neutralization of IL-2 on the activation of CD4⁺ or CD8⁺ cells.

(a) Representative flow cytometric analyses for the experiment shown in Fig. 2e. (b) LN cells of Foxp3^Cre (upper 6 panels) and Foxp3^CreIl2rb^fl/fl (lower 8 panels) mice were unstimulated (US) or stimulated with rmIL-2 (1,000 or 10 U/ml) for 20 min, and intracellular levels of phospho-STAT5 in T_reg (CD4⁺YFP⁺CD25^hi), T naive (YFP⁻CD44^loCD25^lo; CD4⁺ and CD8⁺), and T_eff (YFP⁻CD44^hi; CD25^lo and CD25^hi; CD4⁺ and CD8⁺) cells were analyzed by flow cytometry. Data are representative of two independent experiments with three biological replicates in each experiment.