Supplementary Figure 5: Detection of Trav11Traj18 transcripts and mature DN NKT cells in the DN thymocyte fraction of Rorc–/– mice. | Nature Immunology

Supplementary Figure 5: Detection of Trav11Traj18 transcripts and mature DN NKT cells in the DN thymocyte fraction of Rorc–/– mice.

From: Alternative pathway for the development of Vα14+ NKT cells directly from CD4–CD8– thymocytes that bypasses the CD4+CD8+ stage

Supplementary Figure 5

(a) Trav11Traj18 mRNA expression in sorted CD4+CD8+ DP and CD4-CD8- DN thymic fractions from Rorc-/- mice, as determined by qPCR. Bars show relative expression (mean ± SEM), where Gapdh was used as an internal control. Results are representative from three independent experiments with combined total of three to six mice per group, (n.d.) not detected. (b) Detection of CD1d dimer+ mature NKT cells within DN thymocytes of Rorc-/- mice. Prior to the cell staining, DN thymocytes were purified with AutoMACS by using anti-CD4 and anti-CD8 microbeads. Numbers on plots indicate the percentage and absolute number (mean ± SEM, n=6 mice) of CD1d dimer+ NKT cells within gated DN TCRβ+ cells. Unloaded CD1d staining of DN thymocytes from B6 mice was used as a staining control. Results shown are representative from three independent experiments with two mice per group.

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