Supplementary Figure 6: Lack of Trav11Traj 18 transcripts as well as mature DN NKT cells in the thymus of Ptcra–/– mice. | Nature Immunology

Supplementary Figure 6: Lack of Trav11Traj 18 transcripts as well as mature DN NKT cells in the thymus of Ptcra–/– mice.

From: Alternative pathway for the development of Vα14+ NKT cells directly from CD4–CD8– thymocytes that bypasses the CD4+CD8+ stage

Supplementary Figure 6

(a) The qPCR analysis of Trav11Traj18 mRNA expression in sorted thymic CD4+CD8+ DP, and CD44+CD25- DN1, CD44+CD25+ DN2, CD44-CD25+ DN3, CD44-CD25- DN4 fractions from Ptcra-/- mice. Bars show relative expression (mean ± SEM, n = 3 biological replicates), where Gapdh was used as an internal control. Results are representative from three independent experiments, (n.d.) not detected. (b) Detection of CD1d dimer+ NKT cells within DN thymocytes from B6 and Ptcra-/- mice. Numbers on flow cytometry plots indicate the percentage of CD1d dimer+TCRβ+ NKT cells within electronically gated DN cells. Prior to the cell staining, DN thymocytes were purified with AutoMACS by using anti-CD4 and anti-CD8 microbeads. Results shown are representative from three independent experiments with two mice per group.

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