Supplementary Figure 7: Surface phenotype of NKT cells of DN-stage-thymus origin is similar to that of B6 wild-type stage 3 NKT cells. | Nature Immunology

Supplementary Figure 7: Surface phenotype of NKT cells of DN-stage-thymus origin is similar to that of B6 wild-type stage 3 NKT cells.

From: Alternative pathway for the development of Vα14+ NKT cells directly from CD4–CD8– thymocytes that bypasses the CD4+CD8+ stage

Supplementary Figure 7

Viable 7-AAD-B220-CD1d dimer+TCRβ+ DN thymic NKT cells from B6 and E8III-Cre+Rag2fl/fl mice were analyzed for the expression of CD24, CD44, and NK1.1 surface antigens by flow cytometry. Numbers on the histograms show the percentage of CD24lo DN NKT cells, and numbers on flow cytometry plots represent the percentage of DN NKT cells within the indicated quadrant gates. Results shown are representative from three independent experiments, n = 3 mice per genotype.

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