Supplementary Figure 8: Cytotoxic killing assay with YFP– and YFP+ NKT cells from E8III-Cre+Rosa26-YFP mice. | Nature Immunology

Supplementary Figure 8: Cytotoxic killing assay with YFP– and YFP+ NKT cells from E8III-Cre+Rosa26-YFP mice.

From: Alternative pathway for the development of Vα14+ NKT cells directly from CD4–CD8– thymocytes that bypasses the CD4+CD8+ stage

Supplementary Figure 8

FACS-sorted YFP-negative and YFP-positive thymic DN NKT cells from E8III-Cre+Rosa26-YFP mice were activated with anti-CD3 and anti-CD28 overnight, and then were co-cultured with CellTraceTM Violet labeled YAC-1 target cells at a E/T ratio of 5:1 for 4 hrs. Samples then were FACS analyzed using annexin V and 7-aminoactinomycin D. Bars depict the mean specific cell lysis percentage ± SEM of triplicate samples done in one experiment, and were calculated by the following formula: % cytotoxicity = (target-spontaneous/maximum-spontaneous) ×100.

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