Figure 6: Cytotoxic-lineage characteristics of liver NKT cells of DN-stage origin. | Nature Immunology

Figure 6: Cytotoxic-lineage characteristics of liver NKT cells of DN-stage origin.

From: Alternative pathway for the development of Vα14+ NKT cells directly from CD4–CD8– thymocytes that bypasses the CD4+CD8+ stage

Figure 6

(a) qPCR analysis of genes characteristic of the cytotoxic lineage (vertical axes) in liver DN NKT cells of DN- or DP-stage origin (defined as in Fig. 5a; key); results are presented relative to those of Traj18-Trac (internal control). (b) Flow cytometry of sorted liver DN NKT cells of DN- or DP-stage origin (as in a; effector cells) activated overnight with anti-CD3 and anti-CD28 and then cultured for 4 h together with YAC-1 target cells (labeled with the cell-division tracker dye CellTrace Violet) at an effector cell/target cell ratio of 5:1 and assessed with the apoptosis marker annexin V and the membrane-impermeable DNA-intercalating dye 7-aminoactinomycin D; results are presented as specific cytotoxicity, calculated as follows: percent cytotoxicity = (target cytotoxicity – spontaneous cytotoxicity / maximum cytotoxicity – spontaneous cytotoxicity) × 100. Data are representative of three independent experiments with six to nine mice per group (mean + s.e.m.).

Back to article page