Supplementary Figure 1: Confirmation of the DN-stage origin of NKT cells with the Cd4-Cre+Rosa26-YFP mouse. | Nature Immunology

Supplementary Figure 1: Confirmation of the DN-stage origin of NKT cells with the Cd4-Cre+Rosa26-YFP mouse.

From: Alternative pathway for the development of Vα14+ NKT cells directly from CD4–CD8– thymocytes that bypasses the CD4+CD8+ stage

Supplementary Figure 1

(a) YFP reporter expression within gated viable B220-TCRβ+ splenic CD4+, CD8+, and DN fractions obtained from Cd4-Cre+Rosa26-YFP mice. Numbers on flow cytometry plots show the frequency of cells within indicated gates. (b) Detection of CD1d dimer+ NKT cells within gated YFP-negative and YFP-positive TCRβ+ DN splenocytes from Cd4-Cre+Rosa26-YFP mice. Prior to the cell staining, DN splenocytes were purified by AutoMACS using anti-CD4 and anti-CD8 microbeads. Numbers on flow cytometry plots show the percentage and absolute number of CD1d dimer+ NKT cells within gated YFP-negative and YFP-positive DN TCRβ+ cells. Results shown are from two mice done in one experiment.

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