Figure 1: Deregulated SGK1 expression and activity is associated with infertility and RPL. | Nature Medicine

Figure 1: Deregulated SGK1 expression and activity is associated with infertility and RPL.

From: Deregulation of the serum- and glucocorticoid-inducible kinase SGK1 in the endometrium causes reproductive failure

Figure 1

(a) Timed midsecretory endometrial biopsies from subjects with RPL (n = 9), fertile controls (Fer; n = 9) or women with unexplained infertility (Inf; n = 9) were analyzed for the expression of SGK1 transcripts using quantitative RT-PCR (qRT-PCR). Transcript levels were normalized to the housekeeping gene L19. AU, arbitrary units. (b) Immunostaining for phosphorylated SGK1 (p-SGK1) in timed midsecretory endometrium of fertile women and subjects with RPL or unexplained infertility. Additional images are presented in Supplementary Figure 1. Scale bar, 100 μm. (c) Total protein lysates from snap-frozen endometrial samples (n = 12) subjected to western blot analysis and immunoprobed with antibodies specific to total SGK1 and pSGK1. β-actin was used as a loading control. *P < 0.05; **P < 0.01; ***P < 0.001.

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