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Integrated supplementary information
Supplementary Figure 1 Substantial allele imbalance is present in all three Schaefer et al. animals, raising concerns about the ability to call heterozygous variants accurately.
The allele fraction of Fref is included for reference. For each genome, note the distinct peak at VAF = 0.5.
Supplementary Figure 2 Homozygous variant sites in Fcon, F03 and F05 are highly overlapping.
A) The number and overlap of homozygous variants (with respect to mm10 and Fref) in F03 and Fcon. B) Same as A, but for F05 and Fcon.
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Supplementary Figures 1–2, Supplementary Tables 1–3 and Supplementary Methods (PDF 183 kb)
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Lescarbeau, R., Murray, B., Barnes, T. et al. Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo”. Nat Methods 15, 237 (2018). https://doi.org/10.1038/nmeth.4553
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DOI: https://doi.org/10.1038/nmeth.4553
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