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Different delivery methods—different expression profiles

Nature Methods volume 2, page 241 (2005)Cite this article

A Corrigendum to this article was published on 01 July 2005

To the editor:

Recent publications by several groups of researchers have suggested that small interfering RNAs (siRNA) delivered by lipid-mediated transfection induce both sequence-specific effects1 and broad, class-specific changes in gene expression1,2,3,4. These findings challenge convictions previously held in the RNA interference (RNAi) community that assert virtual sequence specificity of siRNA knockdown, and they bring into question the value of this methodology as a research and therapeutic tool.

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Figure 1: Comparative microarray profiling identifies lipid carrier–mediated gene modulation.

References

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Authors and Affiliations

  1. Dharmacon Research, 2650 Crescent Dr., #100, Lafayette, 80026, Colorado, USA

    Yuriy Fedorov, Emily Anderson, Jon Karpilow, William Marshall & Anastasia Khvorova

  2. Cyto Pulse Sciences, Inc., P.O. Box 609, Columbia, 21045, Maryland, USA

    Alan King

  3. Agilent Technologies, 3500 Deer Creek Rd, MS 25U-7, Palo Alto, 94034, California, USA

    Diane Ilsley

Authors
  1. Yuriy Fedorov
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  2. Alan King
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  3. Emily Anderson
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  4. Jon Karpilow
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  5. Diane Ilsley
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  6. William Marshall
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  7. Anastasia Khvorova
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Corresponding author

Correspondence to Anastasia Khvorova.

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Fedorov, Y., King, A., Anderson, E. et al. Different delivery methods—different expression profiles. Nat Methods 2, 241 (2005). https://doi.org/10.1038/nmeth0405-241

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