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GFP expression in muscle cells impairs actin-myosin interactions: implications for cell therapy

Nature Methods volume 3, page 331 (2006)Cite this article

A Corrigendum to this article was published on 01 June 2006

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To the editor:

One of the major issues of cell transplantation is the in vivo tracking of injected cells to monitor their phenotypic changes and correlate engraftment with function. To this end, GFP and its variants have been most commonly used1,2. Although expression of GFP is considered to be innocuous for the cells, deleterious effects have been reported in in vitro3,4 and in vivo studies5,6, and include the development of dilated cardiomyopathy in mice5. The therapeutic potential of skeletal myoblasts is increasingly investigated in both cardiology and myology, and GFP is often used to better define the fate of transplanted myoblasts. Because nonspecific sarcomeric labeling of GFP occurs in muscle, we investigated the cellular effects of eGFP expression on muscle cell function.

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Acknowledgements

This work was supported by INSERM, and the Association Française Contre les Myopathies (AFM). We thank the Genethon, which is supported by the AFM, in the frame of the Gene Vector Production Network (http://www.gvpn.org) for providing us with lentiviral vectors. We thank G. Butler-Browne and J.-L. Samuel for helpful discussions.

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Author notes

  1. Onnik Agbulut and Catherine Coirault: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry, Université Paris 7, Equipe d'Accueil 300, Paris, France

    Onnik Agbulut, Alexis Huet & Patrick Vicart

  2. Institut National de la Santé et de la Recherche Médicale, Unité 689, Paris, France

    Catherine Coirault

  3. Centre National de la Recherche Scientifique, Montpellier, 2593, France

    Nicolas Niederländer & Michel Puceat

  4. Department of Cardiology, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Université Paris-Descartes, Paris, France

    Albert Hagège

  5. Institut National de la Santé et de la Recherche Médicale, Unité 633, Paris, France

    Albert Hagège & Philippe Menasché

  6. Department of Cardiovascular Surgery, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Université Paris–Descartes, Paris, France

    Philippe Menasché

Authors
  1. Onnik Agbulut
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  2. Catherine Coirault
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  3. Nicolas Niederländer
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  4. Alexis Huet
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  5. Patrick Vicart
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  6. Albert Hagège
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  7. Michel Puceat
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  8. Philippe Menasché
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Correspondence to Onnik Agbulut.

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Agbulut, O., Coirault, C., Niederländer, N. et al. GFP expression in muscle cells impairs actin-myosin interactions: implications for cell therapy. Nat Methods 3, 331 (2006). https://doi.org/10.1038/nmeth0506-331

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