Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Suitability of hCMV for viral gene expression in the brain

Nature Methods volume 4, page 379 (2007)Cite this article

Access through your institution
Buy or subscribe

To the editor:

Viral gene delivery is a powerful tool that is rapidly gaining popularity in experimental neuroscience. I believe that the near future will see rapid expansion of this remarkably versatile and adaptable method. Recent communication by Wickersham et al.1 describes yet another interesting implementation of this technology for retrograde tracing of neurons from their target areas. The key point of the paper is demonstration of a high level of enhanced GFP (EGFP) expression using a rabies virus mutant with glycoprotein gene substituted by EGFP. After injecting the vector into the thalamus, the authors showed many bright EGFP-expressing cortical pyramidal neurons. As a reference, they used a lentivirus with rabies virus envelope glycoprotein in which EGFP expression was driven by human cytomegalovirus (hCMV) promoter and found a very low level of EGFP expression with it.

This is a preview of subscription content, access via your institution

Access options

Access through your institution

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Wickersham, I.R., Finke, S., Conzelmann, K.K. & Callaway, E.M. Nat. Methods 4, 47–49 (2007).

    Article  CAS  PubMed  Google Scholar 

  2. Lonergan, T., Teschemacher, A.G., Hwang, D.-Y., Kim, K.-S. & Kasparov, S. Physiol. Genom. 20, 165–172 (2005).

    Article  CAS  Google Scholar 

  3. Allen, A.M. et al. Hypertension 47, 1054–1061 (2006).

    Article  CAS  PubMed  Google Scholar 

  4. Wang, C.Y. & Wang, S. Gene Ther. 13, 1447–1456 (2006).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. University of Bristol, Bristol, BS8 1TD, UK

    Sergey Kasparov

Authors
  1. Sergey Kasparov
    View author publications

    Search author on:PubMed Google Scholar

Ethics declarations

Competing interests

The author declares no competing financial interests.

Supplementary information

Supplementary Fig. 1

Adenoviral vectors containing hCMV-EGFP expression cassette injected into the nucleus tractus solitarius (NTS) of rats. (PDF 135 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kasparov, S. Suitability of hCMV for viral gene expression in the brain. Nat Methods 4, 379 (2007). https://doi.org/10.1038/nmeth0507-379a

Download citation

  • Issue date:

  • DOI: https://doi.org/10.1038/nmeth0507-379a

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing