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Photobleaching of YFP does not produce a CFP-like species that affects FRET measurements

Nature Methods volume 3, pages 492–493 (2006)Cite this article

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Valentin and colleagues respond:

Verrier & Söling1 and Thaler et al.2 did not observe yellow fluorescent protein (YFP) to cyan fluorescent protein (CFP) photoconversion during YFP photobleaching, questioning our previous results3. To exclude the possibility that photoconversion was specific to our experiments, we tested different microscopes, constructs and cells, including the sample used in Supplementary Figure 2b of the Verrier & Söling report. We detected photoconversion in all cases (Supplementary Fig. 1 online), indicating that it occurred independently of the cell lines, cell preparation and microscope used.

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References

  1. Verrier, S. and Söling, HD. Nat Methods 3, 491 (2006).

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  2. Christopher Thaler, C., Vogel, S.S., Ikeda, S.R. & Chen, H. Nat Methods 3, 491 (2006).

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  3. Valentin, G. et al. Nat. Methods 2, 801 (2005).

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Authors
  1. Guillaume Valentin
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  2. Cèline Verheggen
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  3. Tristan Piolot
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  4. Henry Neel
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  5. Timo Zimmermann
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  6. Maïté Coppey-Moisan
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  7. Edouard Bertrand
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Valentin, G., Verheggen, C., Piolot, T. et al. Photobleaching of YFP does not produce a CFP-like species that affects FRET measurements. Nat Methods 3, 492–493 (2006). https://doi.org/10.1038/nmeth0706-492

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