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Semiconductor nanocrystal conjugates, FISH and pH

Nature Methods volume 2, page 723 (2005)Cite this article

To the editor:

Properties of high luminescence, reduced photobleaching and single-wavelength excitation make quantum dots (QDs) ideal for bioimaging1. Fluorescence in situ hybridization (FISH) applications have been limited for lack of reagents, stable surface treatments2 and optimization. We previously demonstrated QD-FISH detection of DNA probes with (CdSe)/ZnS-streptavidin conjugates3. Here we show pH effects on fluorescence of QD-detected hybridization signals in FISH experiments that are not evident in solution experiments.

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Figure 1: pH dependence of fluorescence intensity in FISH experiments with total genomic DNA detected by Texas red and QDs.

References

  1. Alivisatos, P. Nat. Biotechnol. 22, 47–52 (2004).

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  2. Pathak, S., Choi, S-K., Arnheim, N. & Thompson, M.E. J. Am. Chem. Soc. 123, 4103–4104 (2001).

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  3. Xiao, Y. & Barker, P.E. Nucleic Acids Res. 32, e28 (2004).

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Author information

Authors and Affiliations

  1. Biotechnology Division, DNA Technologies Group, National Institute of Standards and Technology, Gaithersburg, 20899, Maryland, USA

    Yan Xiao & Peter E Barker

  2. Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, 20892, Maryland, USA

    William G Telford

  3. Analytical Chemistry Division, Molecular Spectrometry and Microfluidic Methods Group, National Institute of Standards and Technology, Gaithersburg, 20899, Maryland, USA

    J Christopher Ball & Laurie E Locascio

Authors
  1. Yan Xiao
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  2. William G Telford
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  3. J Christopher Ball
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  4. Laurie E Locascio
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  5. Peter E Barker
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Supplementary information

Supplementary Fig. 1

Supplemental Data Fluorescence intensity stability in FISH signals rescanning by LSC. (PDF 179 kb)

Supplementary Fig. 2

pH dynamics experiments. (PDF 313 kb)

Supplementary Data (PDF 78 kb)

Supplementary Methods (PDF 64 kb)

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Xiao, Y., Telford, W., Ball, J. et al. Semiconductor nanocrystal conjugates, FISH and pH. Nat Methods 2, 723 (2005). https://doi.org/10.1038/nmeth1005-723

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