Supplementary Figure 4: Number and volume of lipofuscin inclusions in microglia increases with age
From: Age-related myelin degradation burdens the clearance function of microglia during aging
(a) Confocal images showing lipofuscin (grey) and microglia (Iba1, red) in a 18 month old wild type mouse. Scale bar: 20µm. (b) Quantification of lipofuscin volume in µm3 in 2, 9 and 24 month old mice (n=4 mice per group, mean +/- s.d., one-way ANOVA, ***P<0.0001, F=54.62, df=113, followed by Bonferroni’s post hoc test indicates significant difference in 9 vs 24 months, ***P<0.0001, t=7.821). (c) Comparison of lipofuscin volume in µm3 in the grey and white matter of 18 and 24 month old mice (n=4 mice per group, mean +/- s.d., two-way ANOVA, brain region effect: **P= 0.0026, F=45.10, followed by Bonferroni’s post hoc test, *P=0.0362, t=3.928, *P=0.0325, t=5.570). Each dot represents the mean value of 40 cells.
