Supplementary Figure 6: Characterization of conditional Rab7 knockout mice | Nature Neuroscience

Supplementary Figure 6: Characterization of conditional Rab7 knockout mice

From: Age-related myelin degradation burdens the clearance function of microglia during aging

Supplementary Figure 6

(a) Strategy for conditional targeting of Rab7 in mice. (b) Timeline diagram for histological analysis of conditional Rab7 knockout mice. (c) Confocal image of cortex of CX3CR1+/CreERT2:Rab7flox/+: tdTomato+ mice is shown. Microglia were visualized with Iba1 in green and the number of Iba1+/tdTomato+ (red) cells were counted one week after tamoxifen injections (for 5 days with 75mg/kg body weight). tdTomato is expressed in more than 90% of the microglia. Scale bar: 300µm. (d) RT-PCR analysis of Rab7 from isolated microglia from Rab7 flox/flox (control) and Rab7flox/flox:CX3CR1+/CreERT2 (Rab7ΔMG) mice. (e) Confocal image of the cortex of control and Rab7ΔMG animals 6 weeks after tamoxifen injections is shown. Arrows point to enlarged lysosomes (Lamp1, red) within microglia (Iba1, green). Scale bar: 20µm. Quantification of number of Lamp1-positive microglia per area (n=4 mice per group, mean +/- s.d., two-way ANOVA, genotype effect: ***P<0.0001, F= 421.5, followed by Bonferroni’s post hoc test, ***P<0.001, *P=0.0425, t=3.115). Each dot represents the mean value of 3 brain slices per mouse.

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