Failure to Replicate an Association of a LINE-1 Element in ERI1 Exoribonuclease Family Member 3 (ERI3) with Schizophrenia

We reported previously that a LINE-1 element in the ERI3 gene was more common among lymphoblastoid blood DNA samples from European-American patients diagnosed with schizophrenia (PDS) than those from controls (Doyle et al, 2017).

We sought to replicate our original finding in a second set of blood DNA samples from PDS and controls. De-identified DNA from EBV-transformed lymphoblastoid cell lines of European-American subjects who met DSM-IV criteria for schizophrenia (n=1665) and controls (n=997) were acquired from the Rutgers University Cell and DNA Repository (Infinite Biologics, Piscataway, NJ, USA). Two microliters of DNA at 20 ng/μl in sterile water were used in genotyping experiments. A Taqman-based allelic discrimination assay was used, as described (Doyle et al, 2017), to genotype these additional PDS and control samples for the presence or absence of the LINE-1 element within ERI3.

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