Supplementary Figure 7: Comparison of sequencing coverage of cytosines in CpG, CHG and CHH context (H can be A, C or T) between T-WGBS and conventional WGBS.

Coverage versus cytosine density in 1-kb windows for WGBS (red) and T-WGBS (blue). The coverage appears stable in genomic regions with 100–400 cytosines, likely because the majority of 1-kb regions has a cytosine density between 10% and 40%. Variation becomes larger in the two extremes of the cytosine density for both T-WGBS and conventional WGBS due to low counts in each category.