Research articles

Microplastics (MPs) are persistent and widely distributed pollutants. Environmental factors cause physical and chemical aging that change their properties. This protocol is a systematic workflow for controlled MP aging and its characterization.

This Protocol Extension describes the infection of adult Drosophila with Pseudomonas aeruginosa by feeding, as opposed to a previous protocol that used needle-pricking or injector-pumping to impose a wound or a direct systemic infection, respectively.

This protocol identifies endogenous biomolecular condensates by combining sucrose density gradient centrifugation with quantitative mass spectrometry, enabling proteome-wide, stimulus-responsive profiling of phase-separating proteins.

This Protocol details the use of enhancer-mediated genomic recording of activity in multiplex (ENGRAM) for molecular recording. This system converts the transient activity of cis-regulatory elements into stable genomic records that can be retrospectively recovered via DNA sequencing.

This Protocol describes a simplified ischemia-free liver transplant procedure, where the portal vein is directly cannulated into the donor portal vein, eliminating the need for an interposition vein.

This protocol details the design principles and procedures for building programmable CRISPRi-based gene circuits, and for testing individual components and the assembled circuits in various plant species using a high-throughput protoplast-based assay.

This protocol details in vitro and in vivo approaches for labeling tumor cells with secreted luciferases to enable precise, longitudinal monitoring of tumor growth in mice, through the analysis of small blood samples.

This protocol describes the synthesis, purification and assembly of stable branched 4-way junction (4WJ) RNA nanoparticles conjugated with multiple hydrophobic chemotherapeutic drug molecules to target specific tumors with minimal side effects.

This Protocol Extension is an adapted version of the authors’ previous INFOGEST static digestion model (INFOGEST 2.0), with an added workflow for the quantification of total protein digestibility, individual amino acid digestibility and digestible indispensable amino acid score.

A protocol for the planning and implementation of magnetoencephalography experiments across nonclinical and clinical settings, including the methodological considerations required for designing multidisciplinary, population-based studies.

This protocol outlines an extracellular vesicle detection approach in which reagent-loaded liposomes fuse with extracellular vesicles directly in patient blood to sensitively detect RNA within the extracellular vesicles.

A protocol for cryogenic 3D correlative focused ion beam milling using an integrated fluorescence light microscope and montage cryo-ET for nonadherent and adherent mammalian cells, as well as primary Drosophila melanogaster neurons.

CellRank enables cell fate mapping from single-cell RNA sequencing data in a consistent and scalable manner, relying on different views of the data such as pseudotime or stemness potential, RNA velocity and experimental time points.

The authors provide a suite of in vitro protocols through which mouse and human pluripotent stem cells, mouse blastocysts and human blastoids can be reversibly induced to enter a diapause-like dormant state via pharmacological inhibition of mTOR

Investigating mechanical forces during angiogenic sprouting by three-dimensional traction force microscopy (TFM) in hydrogel matrices, including steps to analyze and interpret cell–extracellular matrix forces using open-source TFMLAB software.

This protocol isolates lignin from various types of biomass, screens for high-quality lignins, and uses them to directly prepare lignin adhesives without chemical modification, the performance of which is then assessed. It produces lignin adhesives with light colors and superior properties.

This protocol details transsynaptic tracing approaches using retrograde and anterograde viral tracers to map and manipulate neuron–tumor circuits in xenografts, brain organoid models and co-cultures.

Urea, formamide, cyclohexanone oxime and amino acids are vital raw materials. Their synthesis often requires high temperatures and dangerous reagents. This protocol describes an aqueous electrocatalytic approach using inorganic nitrogen sources.

This protocol covers a package for running statistical testing on the temporal dynamics of neural activity data obtained from various functional neural recording modalities.

GPSeq is a genome-wide method for probing the radial organization of the genome in cells or nuclei by progressive in situ digestion of chromatin with a restriction enzyme diffusing inward from the periphery followed by high-throughput sequencing.