Research articles

Here, the authors provide a platform for inducible CRISPR–Cas9 screening to interrogate non-proliferative cellular states.

Protocol for generating spatially patterned, human neural tube- and forebrain-like structures, by using a microfluidic device to impose orthogonal and independent chemical gradients on human pluripotent stem cells.

The simultaneous recording of fast-scan cyclic voltammetry and functional magnetic resonance imaging signals enable the coupling of local tissue oxygen and neurotransmitter dynamics for measuring changes in the brain

The synthesis of complex carbohydrates requires multiple steps, one of which is 1,2-cis-selective glycosylation. This protocol describes an iron-catalyzed, general approach to formation of rather challenging 1,2-cis-aminoglycosidic linkages.

To understand enzymatic redox reaction mechanisms, it is important to investigate the redox behavior at the metal centers. This Protocol describes metalloenzyme attachment to electrodes and how to perform X-ray absorption spectroelectrochemistry.

Optoelectronic devices based on two-dimensional layered thin film semiconductors can be tuned to directly sense and process spatial and temporal information, making them suitable to use as in-sensor computing devices.

NanoVar is a structural variant caller for low-depth long-read sequencing data, allowing repeat element annotation of non-reference insertion variants. This is a comprehensive guide, from quality assessment of raw data to downstream analysis.

This protocol for imaging-based highly multiplexed in situ profiling of the spatial transcriptome uses three methods: antibody-based protein comapping with STARmap PLUS, ribosome-bound mRNA mapping with RIBOmap and spatiotemporal transcriptome mapping with TEMPOmap.

A technique for ultrasound-induced luminescence imaging, which uses a dual-stage energy conversion mechanism to enhance the performance of luminescence.

In the virus-encoded CRISPR-based direct readout system, single-guide RNA libraries are expressed directly from the genomes of viruses so that single-guide RNA abundance at distinct stages of the viral infection cycle provides direct and quantitative readout of gene-perturbation effects on viral propagation.

Mehl and colleagues present a protocol for selecting aminoacyl-tRNA synthetase (RS)/tRNA pairs from a publicly available MaPylRS active site mutant library for specific and efficient incorporation of noncanonical amino acids into proteins.

Aspiration-assisted bioprinting is a versatile biofabrication technique that enables the precise and selective patterning of biologics, such as tissue spheroids and organoids, addressing the limitations of conventional bioprinting techniques.

This Protocol provides detailed instructions for performing NAP-seq, an RNA sequencing technique for profiling full-length sequences of noncapped RNAs with various terminal modifications at single-nucleotide resolution.

This protocol describes a versatile strategy to generate structurally and functionally programmable hydrogels by using biological membranes as cross-linkers. This enables properties of tissues like muscle and skin to be mimicked.

Ghatak and colleagues describe a protocol for labeling cell-type-specific exosomes via targeted expression of reporters, isolation from mouse skin tissues by immunomagnetic separation and characterization using established assays.

In this Protocol, three dual adeno-associated viral strategies for delivery of large transgenes are presented—by reconstitution at the genomic, transcript or protein level—together with instructions for the design, production and evaluation of dual adeno-associated viral vectors.

This protocol presents a versatile approach for the expression and purification of diverse recombinant protein complexes for structural studies, leveraging transient expression in Nicotiana benthamiana leaves to achieve high yield and purity.

This protocol extension guides users in identifying candidate drug metabolites without prior knowledge by analyzing tandem mass spectrometry data in the web-based molecular networking tool GNPS2.

The protocol provides instructions for the use of FunFit, a software package to quantitatively map smooth topographic landscapes and improve the analysis, reproducibility and processing of thermal scanning-probe lithography data.

This is a Protocol for synthesizing transition metal-sensitized lanthanide luminescent nanoparticles and for preparing a library of advanced luminescent materials that enable increases in brightness in near-infrared emission.