Supplementary Figure 5: High consistency, 97.8% and 98.3%, between T-WGBS and conventional WGBS methylation data from two human blood samples, M and K, respectively.

For each sample, two lanes of T-WGBS data and three lanes of conventional WGBS were compared. (a,b) Methylation levels were calculated based on scanning windows of 5 CpGs with at least 30-fold coverage and displayed on density plots (a) and scatter plots (b). The dotted lines mark the 0.2 difference between the x and y axes. Consistency values were calculated by subtracting from 100% the respective numbers in the corners of the density plots displayed in (a); these numbers indicate the percentage of data points that fall above/below the envelope marked with dotted lines. The r values in (b) indicate Pearson correlation.