Supplementary Figure 2: Genotyping: exemplary results of a T7 endonuclease I assay.
From: Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA
Wild-type controls (wt) harbor only the full length PCR product (open triangle), whereas heterozygous mutants (het) show the additional presence of two digestion products (filled triangles) obtained by the cleavage of heteroduplex molecules within the TALEN target region.
