Supplementary Figure 3: Calibration of the wire.
From: Generating suspended cell monolayers for mechanobiological studies
(a) Composite image of the wire position before and after loading. The right extremity of the wire is threaded into a glass capillary that is maintained horizontal. The left extremity is left free. Upon loading, a small mass of plasticine is added to the free extremity of the wire. The total length of the wire Lw, the point of loading y, and the deflection d of the wire following loading are indicated on the image. Scale bar = 5mm. (b) Force plotted as a function of flexural rigidity 6Id/(3Lw-y)y2. Force is given in 10-4 N. Flexural rigidity is given in 10-15 m2. Experimental data points are indicated by black dots. A straight line is fitted to the experimental data points and its slope is the elastic modulus of the wire.
