Supplementary Figure 11: Thioflavin T assays of purified α-synuclein 8.
From: Chemical synthesis of proteins using peptide hydrazides as thioester surrogates
The sample with 10 μM (containing 20 mM Tris, 150 mM NaCl, pH 7.5) is incubated at 37° C. Aliquots are taken and analyzed by ThT fluorescence (450 nm/482 nm) at the indicated time points. The experiments are performed in triplicate.
