Supplementary Figure 4: RP-HPLC analysis of α-synuclein(30-68, A30C)-NHNH2 running on a C4-column.
From: Chemical synthesis of proteins using peptide hydrazides as thioester surrogates
The linear gradient for analytical HPLC: 5% buffer B in buffer A to 95% B in A over 30 min at rt (Left: the crude and Right: purified).
