Supplementary Figure 1: Diffusion profile of Sulforhodamine 101 (SR101) when ejected from the tip of a micropipette.

(A) Schematic of fluorescence detection position. Circles in different colors denote different distances from the tip of the micropipette. Scale bar, 50 μm. (B) Quantitative analysis of SR 101 fluorescence intensities. Fluorescence intensities at three different points on each circle were collected and used for data analysis. Data were normalized to the average fluorescence intensity measured at the position 15 μm from the source after SR101 ejection for 10 min. The arrow marks the time point when the pulsatile application of SR101 was started. Error bars indicate s.e.m. (C) The diffusion profile of SR101 around ∼300 μm from the micropipette tip at different time points is illustrated in a three dimensional surface plot, which was generated using ImageJ with the Interactive 3D Surface Plot plugin. X axis and Y axis indicate distance from the tip of micropipette. Note that the diffusion profile of SR 101 is relatively stable 5 min after its application up to more than 30 min.