Supplementary Figure 2: Applications of VIGS to study gene function in various plant processes.
From: Tobacco rattle virus–based virus-induced gene silencing in Nicotiana benthamiana

Some of the possible studies that can be performed on the silenced plants are given in this figure. VIGS has been used to dissect developmental processes like root and leaf development, meristem differentiation, reproductive organ development, the mechanism of symbiotic interaction with microbes, PAMP or effector-induced defense, nonhost disease resistance, nematode resistance, Agrobacterium-mediated T-DNA transfer, and abiotic stress tolerance in plants.