Supplementary Figure 2: Design of PCR primers for genotyping of knock-in mutations in rat genome.
From: CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos
(a) A primer pair (F and R) is designed outside of the homology arms of the ssODN. (b) Two primer pairs (5'F/5'R and 3'F/3'R) are designed. For each pair, one primer is located in the GOI (the inserted fragment). The other primer is located in rat genome outside the homology arms (left arm or right arm). About 400 bp extension of both left and right arm of the donor plasmid can be used as the positive control for testing the primers (optional).
