Supplementary Figure 3: Generation of double mutant rats by co-injection of Cas9/sgRNAs targeting the Fah and Il2rg loci. | Nature Protocols

Supplementary Figure 3: Generation of double mutant rats by co-injection of Cas9/sgRNAs targeting the Fah and Il2rg loci.

From: CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos

Supplementary Figure 3

Detection of mutations in F0 rats generated by injection of Cas9/sgRNAs targeting Fah (a) and Il2rg (b) after T7EI digestion using PCR products amplified from RNA-injected F0 rat tail genomic DNA. Double mutant rats are indicated by arrowheads. M, DNA marker. (c) DNA Sequence of Fah and Il2rg loci double mutant rats. The target sites are in blue with PAM in red.

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