Supplementary Figure 4: Anticipated results for precise knock-in by ssODNs.
From: CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos
(a) A diagram of Cas9 nickase induced precise gene editing. The sequence of the target site and the ssODNs are listed. (b) Typical sequences of the founders after RNA/DNA injection. The PCR products were amplified with the primers outside of the homology region indicated in (a). Sequences were determined by DNA sequencing of individual TA clones of PCR products. The sgRNA target is underlined, the EcoRI site is in red and the LoxP site is in italics. (c) An example of a DNA sequencing result for precise HR.
