Supplementary Figure 9: Examples showing partial and full coverage of an embryo in plane and volume.
From: Dual-view plane illumination microscopy for rapid and spatially isotropic imaging
a. Shows maximum intensity projections of an embryo which is partially covered (left and middle) and fully covered (right) in X-Y plane. The Y-galvo offset can be changed to bring a partially-covered embryo into full coverage, as shown on the right. b. Maximum-intensity projection of a volume resliced in Z, showing partial (left) and full (right) coverage of an embryo in the Z-direction. If the embryo is only partially-covered in Z, move the diSPIM module until the embryo is fully covered.
