Supplementary Figure 4: Fine adjustment of light sheets, as viewed in the bottom camera.
From: Dual-view plane illumination microscopy for rapid and spatially isotropic imaging
a. The left-most sheet is tilted. b. Rotating the scanner associated with this sheet corrects the tilt, but the two sheets are offset vertically with respect to each other. c. Adjusting the 'Y galvo offset' parameter in the diSPIM Control.vi correctly aligns the vertical offset of the two sheets, but they are still offset in the horizontal direction. d. Bringing the diSPIM module down, towards the coverslip, moves the sheets together until they are aligned. The sample is a coverslip coated with yellow-green fluorescent beads. Scalebars: 200 μm.
