Supplementary Figure 3: Spectra mismatching and poor ladder detection. | Nature Protocols

Supplementary Figure 3: Spectra mismatching and poor ladder detection.

From: Antibody-based detection of protein phosphorylation status to track the efficacy of novel therapies using nanogram protein quantities from stem cells and cell lines

Supplementary Figure 3

Assay data showing total CrkL antibody probed against K562 cell lysate with no TKI reatment. Spectra shown represent technical replicates from the same sample. a, mismatched data example; correct standard ladder pI associations applied to the black trace, and incorrect associations applied to the blue trace. Red dotted lines indicate matching peaks and the extent of peak offset in the blue trace as a result of poor ladder detection. b, manual alteration of this error results in perfect alignment.

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