Supplementary Figure 1: Primer organization layout.
From: Multiplexed locus-specific analysis of DNA methylation in single cells
The high-throughput nature of the assay requires systematic primer organization. We pre-aliquot 50 µM primer mixes as depicted: For each locus/target (1, 2,..., 24) the two primer combinations (forward-short/reverse [green wells] and forward-long/reverse [blue wells]) are arranged pairwise on a 96-well plate. Each row (A-D) is then systematically transferred to the Assay Inlet ports of the Fluidigm array. Photo courtesy of Fluidigm Corporation.
