Supplementary Figure 1: Control experiment for Figure 2.
From: High-throughput microfluidics to control and measure signaling dynamics in single yeast cells
Figure 2 shows a typical experiment for strain EY2967/ASH189 in response to six 5-min pulses of 690 nM 1-NM-PP1 separated by 10- min intervals. This figure shows the same plots with the same axes for a control experiment without 1-NM-PP1 treatment. As can be seen, in the absence of 1-NM-PP1 treatment, no gene expression is observed.
a) Msn2 translocation dynamics. In this control experiment, no 1-NM-PP1 is added, so no Msn2-mCherry activation is observed. Raw data (black dots) and errorbars (standard deviation) are from 101 single cells and the red line shows a fit to the data.
b)-c) Single cell time traces of the YFP (b) and CFP (c) gene expression reporters. Raw, unsmoothed data is shown. As can be seen, in the absence of 1-NM-PP1 treatment, no gene expression is observed.
d) By following both CFP and YFP gene expression dynamics in the same single cell, their co-variance can be computed. Each dot in the scatterplot is the max CFP and YFP from the same single cell.
