Supplementary Figure 1: Irgasan (Triclosan®) and chlroamphenicol select against Pseudomonas aeruginosa, which may lead to protocol failure.

Bacteria from replicate biparental bacterial matings were suspended in 1.0 ml phosphate buffered saline (Step 36A.ix), then divided into three equal aliquots and plated on Vogel-Bonner minimal medium (VBMM) agar, or lysogeny broth (LB) agar containing 25 μg/ml irgasan (Irg) or 10 μg/ml chloramphenicol (Cm). All agar contained 60 μg/ml gentamicin (Gm) to select for merodiploids. Solid lines and error bars represent the mean number and standard deviation of P. aeruginosa merodiploids recovered after conjugation. By comparison to nutritional selection on VBMM agar, the use of Irg and Cm in selective LB agar media reduced the number of merodiploids recovered during selection.