Supplementary Figure 4: The fluorescence of the reporter gene can be enhanced by immunostaining with a specific antibody. | Nature Protocols

Supplementary Figure 4: The fluorescence of the reporter gene can be enhanced by immunostaining with a specific antibody.

From: Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe

Supplementary Figure 4

(a) Confocal image of EGFP fluorescence in a coronal section of a mouse neocortex at P7 after in utero transfection (at E15.5) in an experiment that resulted in low transfection efficiency. (b) Confocal image of the same slice after immunostaining with anti-GFP antibody (Abcam, cat. no. Ab13970; concentration 1:1000). Images in (a) and (b) were acquired using a confocal microscope with the same parameters. Scale bar: 100 µm.

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