Supplementary Figure 1: Traditional electronic gating strategy for analysing cell death based on A5-FITC and 7-AAD.
From: Monitoring the progression of cell death and the disassembly of dying cells by flow cytometry
a, Flow cytometry analysis showing the traditional two-stage electronic gating strategy used to identify viable cells, A5+ early apoptotic cells and necrotic cells. b, Flow cytometry analysis displaying each type of cells gated according to a has distinctive levels of A5-FITC and 7-AAD staining. Human Jurkat T cells were induced to undergo apoptosis by anti-Fas treatment (31.25 ng ml-1, 4 h). Samples were stained with A5-FITC (1 in 200 dilution), 7-AAD (1 μg ml-1) and TO-PRO-3 (0.5 μM) in 1x A5 Binding Buffer. 30,000 events (cells and cell fragments) were recorded by the FACSCanto II flow cytometer and resultant data analysed by the FlowJo 8.8.6 software. The TO-PRO-3 parameter was not used during data analysis.
