Supplementary Figure 3: Representative images of typical particles in each stage of cell death.
From: Monitoring the progression of cell death and the disassembly of dying cells by flow cytometry
ImageStream analysis of particles gated using the strategy as described in Poon et al., Nature 507, 329–334 (2014). Human Jurkat T cells were induced to undergo apoptosis by anti-Fas treatment (250 ng ml−1, 4 h). Samples were stained with A5-FITC (1 in 200 dilution), 7-AAD (1 μg ml−1) and TO-PRO-3 (0.5 μM) in 1x A5 Binding Buffer. Cells and cell fragments were recorded by the ImageStreamX Mark II and resultant data analysed by the IDEAS software. The data shown were previously published in Poon et al., Nature 507, 329–334 (2014).
