Supplementary Figure 1: Linearity of fluorescence detection subsystem | Nature Protocols

Supplementary Figure 1: Linearity of fluorescence detection subsystem

From: Quantifying macromolecular interactions in living cells using FRET two-hybrid assays

Supplementary Figure 1

(a) Fluorescence intensity measured through the YFP cube is linearly proportional to the concentration of Alexa Fluor 514 dye. This result illustrates the linearity of the PMT detector enabling robust estimation of number of acceptor molecules. (b) Fluorescence intensity measured through the CFP cube is also linearly proportional to the concentration of Proflavin dye.

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