Supplementary Figure 7: Data input field of the RD-value (CFP) and the RA-value (YFP) sheets.
From: Quantifying macromolecular interactions in living cells using FRET two-hybrid assays
Raw data are pasted into the respective columns on the left hand side from measurements in (a) CFP-only or (b) YFP-only expressing cells using CFP- FRET- and YFP-cubes at a distinct HV gain. Background subtraction and RA value calculation is performed automatically. (c) The mean RD and RA value, resp., is calculated on the right hand side (gray panel). Statistical values (Standard deviation of the mean (SEM), Minimum (Min) and Maximum (Max)) provide a useful tool to assess the variability of the values. Note that only the mean RD1 and RA1 values are used for subsequent analysis.
