Supplementary Figure 9: Dimer analysis sheet.
From: Quantifying macromolecular interactions in living cells using FRET two-hybrid assays
(a) Columns D-G list background subtracted raw data from the “Dimer” sheet. Data for individual cells are collected separately for a given dimer as indicated in Column B. RA1 and RD1 values are automatically transferred from “RD-value (CFP)” and the “RA-value (YFP)” sheets (grey panel on the top, left). Columns H-P comprise calculations as outlined in Procedure Step 97. (b) The graph (column K plotted against column L) depicts data points for individual cells. For each dimer, cells cluster together. In the table below, the mean values of EA and ED for each dimer as well as mean values of the x- and y- coordinates (from columns M and N) are indicated. (c) The latter can be plotted in a new graph and analyzed by a linear regression line. In this template, the regression line is automatically computed and the resulting equation displayed in the graph. Note, that the y-intercept and the slope of this line is copied to cell T56 and T57 respectively for further analysis.
