Supplementary Figure 3: Upper and lower detection levels for IDAA amplicons in ABI 3130 and 3500 instruments. | Nature Protocols

Supplementary Figure 3: Upper and lower detection levels for IDAA amplicons in ABI 3130 and 3500 instruments.

From: Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis

Supplementary Figure 3

(a) Various amounts of a wt IDAA amplicon from a non-edited control sample were analyzed in ABI 3130 and 3500 instruments. When the loaded amplicons exceed the dynamic range, a smaller peak artifact often appears 20-30 bp ahead of the true signal (indicated by asterisk in the upper 3130 panel). (b) Zoom in on the peaks boxed in (a). The lower 3130 panel illustrates that also below the dynamic range, a specific signal can be discriminated from background signals.

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