Supplementary Figure 4: IDAA reveals the indel signature of a given gRNA, TALEN or ZFN. | Nature Protocols

Supplementary Figure 4: IDAA reveals the indel signature of a given gRNA, TALEN or ZFN.

From: Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis

Supplementary Figure 4

IDAA profiles for two independent experiments using the same construct of FP-linked CRISPR/Cas9 (a,b), TALENs (c) or ZFNs (d), targeting various loci in K562 or HEK293 cells, as indicated. Prior to analysis, the nuclease-transfected cells were subjected to bulk FACS for the top 30% most fluorescent cells. The size and frequency of selected indels are indicated. IDAA profiles were generated in an ABI 3130 instrument.

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