Supplementary Figure 7: Lateral resolutions of confocal microscopy and STED.

Full Width at Half Maximum (FWHM) of Point Spread Function (PSF) is defined as Lateral resolution. 0%, confocal fluorescence microscopy only; 30%, 30 % depletion laser power of 270 mW (reduced to ~21.6 mW at the objective lens); 60 %, 60 % depletion laser power of 540 mW (reduced to ~43.2 mW the objective lens); 90%, 90 % depletion laser power of 810 mW (reduced to ~64.8 mW at the objective lens). Mean + s.e.m.; n= 11 cells (each data point is an average value from multiple GFP-H2B measurements near the DHFR gene locus in a cell); the same cells were imaged by confocal microscopy first and then by STED. 3 independent experiments. P <0.001 between data from any two laser powers. The line is a fit from the inverse square root law.